Research Articles

• Risman, R.A., Belcher, H.A., Ramanujam, R.K., Weisel, J.W., Hudson, N.E., Tutwiler, V. Comprehensive Analysis of the Role of Fibrinogen and Thrombin in Clot Formation and Structure for Plasma and Purified Fibrinogen Biomolecules. 2024 Feb 16;14(2), 230. PMID: 38397467. PMCID: PMC10886591.
• Belcher, H.A., Guthold, M., Hudson, N.E. What is the diameter of a fibrin fiber? Res Pract Thromb Haemost. 2023 July 19;7(5):1-11. PMID: 37601015. PMCID: PMC10439396.
• Pinelo, J.E.E., Manandhar, P., Popovic, G., Ray, K., Tasdelen, M.F, Nguyen, Q., Iavarone, A.T., Offenbacher, A.R., Hudson, N.E., Sen, M. Systematic mapping of the conformational landscape and dynamism of soluble fibrinogen. J Thromb Haemost. 2023 Jun;21(6):1529-1543. PMID: 36746319. PMCID: PMC10407912.
• Stoll E.G., Cone, S.J., Lynch, S.R., Fuguay, A.T., Bannish, B.E., Hudson, N.E. Fluorescent microspheres can affect in vitro fibrinolytic outcomes PLoS One. 2023 Apr 7;18(4):e0284163. PMID: 37027378. PMCID: PMC10081780.
• Risman, R.A., Kirby, N.C., Bannish, B.E., Hudson, N.E., Tutwiler, V.T. Fibrinolysis: An Illustrated Review Res Pract Thromb Haem. 2023 Feb 17;7(2):100081. PMID: 36942151. PMCID: PMC10024051.
• Belcher, H.A., Litwa, K., Guthold, M., Hudson, N.E. The Applicability of Current Turbidimetric Approaches for Analyzing Fibrin Fibers and Other Filamentous Networks Biomolecules. 2022 June 9;12(6):807. PMID: 35740932. PMCID: PMC9221518.
• Bonazza, K., Iacob, R., Hudson, N.E., Li, J., Lu, C., Engen, J.R., Springer, T.A. Von Willebrand factor A1 domain stability and affinity for GPIbα are differentially regulated by its O-glycosylated N- and C-linker eLife. 2022 May9;11:e75760. PMID: 35532124. PMCID: PMC9084892.
• Lynch, S.R., Laverty, S.M., Bannish, B.E., Hudson, N.E. Microscale structural changes of individual fibrin fibers during fibrinolysis. Acta Biomaterialia. 2022 Mar 15;141:114-122. PMID: 35007782. PMCID: PMC8898298.
• Popovic, G., Kirby, N.C., Dement, T.C., Peterson, K.M., Daub, C.E., Belcher, H.A., Guthold, M., Offenbacher, A.R., Hudson N.E. Development of Transient Recombinant Expression and Affinity Chromatography Systems for Human Fibrinogen. IJMS. 2022 Jan 19;23(3). PMID: 35162976. PMCID: PMC8835685.
• Bannish, B.E., Hudson N.E. The Utility and Potential of Mathematical Models in Predicting Fibrinolytic Outcomes. Current Opinion in Biomedical Engineering. 2021 Dec;20. PMID: 34957356. PMCID: PMC8694003.
• Cone S.J., Fuquay A.T., Litofsky J.M., Dement T.C., Carolan C.A., Hudson N.E. Inherent fibrin fiber tension propels mechanisms of network clearance during fibrinolysis. Acta Biomaterialia. 2020 April 15;107:164-177. PMID: 32105833. PMCID: PMC7160043.
• Hudson, N.E. Biophysical Mechanisms Mediating Fibrin Fiber Lysis Biomed Research International, 2017. 1-17 PMID: 28630861. PMCID: PMC5467299.
• Lin, F-Y, Zhu, J. Eng, E.T., Hudson, N.E., Springer, T.A. β-subunit binding is sufficient for ligands to open the integrin αIIbβ3 headpiece Journal of Biological Chemistry, 219(9): 4537-46, 2015. PMID: 26631735. PMCID: PMC4813479.
• Kim, J.*, Hudson, N.E.*, and Springer, T.A. Force-induced Switching to a Faster On-rate and Modulation by Patient Gain-of-function Mutations in Von Willebrand Factor Proc Natl Acad Sci USA, 112(15):4648-53, 2015. PMID: 25810255. PMCID: PMC4403213. * Authors contributed equally to this work.
• Bucay, I., O'Brien, E.T., Wulfe, S.D., Superfine, R., Wolberg, A.S., Falvo, M.R., Hudson, N.E. Physical Determinants of Fibrinolysis in Single Fibrin Fibers PLoS ONE, 10(2): e0116350, 2015. PMID: 25714359. PMCID: PMC4340865.
• Dong, X., Hudson, N.E., Lu, C., Springer, T.A. Structural Determinants of Integrin β-subunit Specificity for Latent TGF-β Nature Structural & Molecular Biology, 21(12), p. 1091-1096, 2014. PMID: 25383667. PMCID: PMC4717663.
• Hudson, N.E., Ding, F., Bucay, I., O'Brien, E.T., Gorkun, O.V., Superfine, R., Lord, S.T., Dokholyan, N.V., Falvo, M.R. Sub-millisecond Elastic Recoil Reveals Molecular Origins of Fibrin Fiber Mechanics Biophysical Journal, 104(12), p. 2671-2680, 2013. PMID: 23790375. PMCID: PMC3686331.
• Houser, J.R.*, Hudson, N.E.*, O'Brien, E.T., Superfine, R., Lord, S.T., and Falvo, M.R. Evidence that αC Region is Origin of Low Modulus, High Extensibility, and Strain Stiffening in Fibrin Fibers Biophysical Journal, 99(9) 3038-3047, 2010. PMID: 21044602. PMCID: PMC2965937. * Authors contributed equally to this work.
• Hudson, N.E., Houser, J.R., O'Brien, E.T., Taylor Jr., R.M., Superfine, R., Lord, S.T., and Falvo, M.R. Stiffening of Individual Fibrin Fibers Equitably Distributes Strain and Strengthens Networks, Biophysical Journal, 98(8) p.1632-1640, 2010. PMID: 20409484. PMCID: PMC2856168.

Conference Abstracts

34. Belcher, H., Risman, R., Ramanujam, R., Tutwiler, V., Hudson, N. Multi-technique study of the role of fibrinogen and thrombin in clot formation and structure , ISTH 2023, Montreal, Canada, June, 2023.

33. Popovic, G., Kirby, N., Dement, T.C., Peterson, K.M., Daub, C.E., Belcher, H.A., Offenbacher, A.R., Hudson, N.E. Towards a Robust Recombinant Expression and Purification Process for Human Fibrinogen , 26th International Fibrinogen Research Society Worskshop, Jongny sur Vevey, Switzerland, June, 2022.

32. Lynch, S.R., Laverty, S.M., Bannish, B.E.,Hudson, N.E. Microscale Mechanisms Regulating Fibrin Clearance During Fibrinolysis , Abstract for the AHA Vascular Discovery: from Genes to Medicine Scientific Sessions 2022, Seattle, WA, May, 2022.

31. Peterson, K., Kirby, N., Popovic, G., Offenbacher, A.R., Hudson, N.E. Novel Affinity-based Fibrinogen Purification Strategies, Abstract for the 2021 BMES Annual Meeting, Orlando, FL, October, 2021.

30. Daraei, A., Dement, T.C., Belcher, H.A., Hudson, N.E., Guthold, M. Fibrinogen α-C Connector is a Major Contributor to the Mechanical Strength of Fibrin Fibers , Abstract for the International Fibrinogen Research Society Mini-Symposium, June, 2021.

29. Cone, S.J., Lynch, S.R., Dement, T.C., Fuquay, A.T., Litofsky, J.L., Carolan, C.A., Bannish, B.E., Hudson, N.E.Quantifying the Inherent Tension in Fibrin Fibers and Understanding Its Role in Fibrinolytic Clearance , Abstract for the International Fibrinogen Research Society Mini-Symposium, June, 2021.

28. Belcher, H.B., Guthold, M., Hudson, N.E.Assessing Current Turbidimetric Approaches in Determining Fibrin Fiber Properties , Abstract for the International Fibrinogen Research Society Mini-Symposium, June, 2021.

27. Lynch, S.R., Bannish, Hudson, N.E.Mechanisms of Fibrinolysis of Fibrin Fibers , Abstract for the International Fibrinogen Research Society Mini-Symposium, June, 2021.

26. Hudson, N.E.Inherent Polymer Tension Regulates the Enzymatic Breakdown of Blood Clots , Abstract for the Endeavor Research 2nd Webinar on Chemistry, June, 2021.

25. Popovic, G., Hudson, N.E., Offenbacher, A.R.Measuring the Effects of Fibrinogen Glycosylations on Fibrin Polymerization using Turbidity , Abstract for the SoDM Celebration of Research and Scholarship (CORAS) 2020, Greeenville, NC, Feb., 2021.

24. Belcher, H.B., Guthold, M., Hudson, N.E.The Validity of Current Turbidimetric Approaches in Determining Fibrin Diameter , Abstract for the SoDM Celebration of Research and Scholarship (CORAS) 2020, Greeenville, NC, Feb., 2021.

23. Lynch, S.R., Bannish, B.E., Hudson, N.E.Resolving Structural Changes In Fibers During Fibrinolysis , Abstract for the 65th Annual Biophysical Society Meeting, Boston, MA, Feb., 2021.

22. Lynch, S.R., Bannish, B.E., Hudson, N.E.Resolving Structural Changes During the Digestion of Fibrin Fibers , Abstract for the Triangle Cytoskeleton Meeting 2020, Raleigh, NC, Nov., 2020.

21. Cone, S.J., Dement, T.C., Litofsky, J.L., Fuquay, A.T., Carolan, C.A., Hudson, N.E.Unexpected, Tension-Dependent Mechanisms Govern Network Clearance During Fibrinolysis , Abstract for the 64th Annual Biophysical Society Meeting, San Diego, CA, Feb., 2020.

20. Daraei, A., Dement, T.C., Hudson, N.E., Guthold, M. Intrinsically Unfolded Alpha-C Region Of Fibrinogen is Major Contributor to Mechanical Strength of Fibrin Fibers , Abstract for the 64th Annual Biophysical Society Meeting, San Diego, CA, Feb., 2020.

19. Huda, F., Abdel-Salam, T.M., Hudson, N.E., Vahdati, A. Investigation of the interplay between blood and thrombus mechanical properties: A 3D fluid-solid interaction model Abstract for the Summer Biomechanics, Bioengineering and Biotransport Conference (SB3C2019), Seven Springs, PA, June, 2019.

18. Hudson, N.E., Fuquay, A.T., Cone, S.J. Fibrin Density and Tension Regulates Fibrinolytic Susceptibility Abstract for the 63rd Annual Biophysical Society Meeting, Baltimore, MD, March, 2019.

17. Gough, C.R., Dement, T.C., Spangler, S., Hudson, N.E. Characterization of Engineered Fibrinogen to Understand the Role of the & αC Region in Fibrin Mechanical Properties and Polymerization during Haemostasis, Abstract for the BMES Annual Meeting, Atlanta, GA, October, 2018.

16. Cone, S.J., Fuquay, A.T., Hudson, N.E. Mechanics Governing Fibrin Fibers During Dissociation of Blood Clots, Abstract for the 11th Biennial Carolina Biophysics Symposium, Chapel Hill, NC, October, 2018.

15. Dement, T.C., Spangler, S., Gought, C.R., Hudson, N.E. Probing the αC Connector's Role in Fibrin Fiber Mechanics, Abstract for the 11th Biennial Carolina Biophysics Symposium, Chapel Hill, NC, October, 2018.

14. Fuquay, A.T., Hudson, N.E. Elongation of fibrin fibers during fibrinolysis, Abstract for the 25th International Fibrinogen & 3rd Factor XIII Workshop, Winston-Salem, NC, June, 2018.

13. Hudson, N., Breaking Down Blood Clots One Fiber at a Time, Abstract for the 61st Annual Meeting of the Biophysical Society, New Orleans, LA, Feb., 2017.

12. Hudson, N., Kim, J., Sprinter, T.A. On-Rate Switching under Force Increases the Binding of von Willebrand Factor A1 to GPIbα, Abstract for the 60th Annual Meeting of the Biophysical Society, Los Angeles, CA, Feb., 2016

11. Bucay, I., Wulfe, S.D., Hudson, N.E., O'Brien, E.T., Falvo, M.R., Determinants of Fibrinolysis in Single Fibrin Fibers, Abstract for the 58th Annual Meeting of the Biophysical Society, San Francisco, CA, Feb., 2014.

10. Yang, D., Halvorsen, K., Hudson, N.E., Wong, W. Study of the von Willebrand Factor A2 Domain using a Miniature Centrifuge Force Microscope and Molecular Dynamics Simulations, Abstract for the 58th Annual Meeting of the Biophysical Society, San Francisco, CA, Feb., 2014.

9. Hudson, N.E., Measuring conformational changes in integrin alpha?V beta?6, Abstract for the Single Molecule Biophysics 2013 Conference, Aspen, CO, Jan., 2013.

8. Hudson, N.E., Bucay, I., Gorkun, O.V., O'Brien, E.T III, Superfine, R., Falvo, M.R. Snapping Recoil: Fibrin Fibers Regain Their Tension within Milliseconds of Being Plucked, Abstract for the XXIInd International Fibrinogen Workshop, Brighton, UK, July, 2012.

7. Lord, S.T., Ping, L., Hudson, N.E., Falvo, M.R. Molecular Mechanisms that Control Clot Structure: Exploring the αC region with a human/chicken hybrid fibrinogen, Abstract for the XXIII Congress of the International Society on Thrombosis and Haemostasis 57th Annual SSC Meeting, ICC Kyoto, Kyoto, Japan, Jul. 23-28, 2011.

6. Hudson, N., Ping, L., Olusesi, O., O'Brien, E.T III , Superfine, R., Lord, S., Falvo, M. Investigating the Role of the Alpha-C domain in Fibrin Fiber Mechanics, Abstract for the 55th Annual Meeting of the Biophysical Society, Baltimore, MD, Mar. 5-9, 2011.

5. Hudson, N., Peng, H., O'Brien, E.T III , Lord, S., Superfine, R., Falvo, M. Fibrin Fibers Exhibit Two Distinct Temporal Regimes of Recoil Dynamics, Abstract for the 55th Annual Meeting of the Biophysical Society, Baltimore, MD, Mar. 5-9, 2011.

4. Hudson, N.E., Olusesi, O., O'Brien, E.T., Lord, S.T., Superfine, R., Lord, S., Falvo, M.R., Strain Stiffening Fibers Strengthen Fibrin Networks, Abstract for the Materials Research Society Spring Meeting, San Francisco, CA April 5-9, 2010.

3. Hudson, N., Millard, D., Houser, J., O'Brien, E.T III, Lord, S.T., Superfine, R., Falvo, M.R. AFM Manipulation of Small Fibrin Networks, Abstract for the 53rd Annual Meeting of the Biophysical Society, Boston, MA., Feb. 28 - Mar. 4, 2009.

2. Falvo, M.R., Hudson, N., Millard, D., O'Brien, E.T III, Superfine, R. AFM Mechanical Studies of a Novel Form of The Biopolymer Fibrin: Elastomeric Sheets, Abstract for the 53rd Annual Meeting of the Biophysical Society, Boston, MA., Feb. 28 - Mar. 4, 2009.

1. Hudson N., Millard, D., Houser, J., O'Brien, E.T., Lord, S., Superfine, R., Falvo, M. The role of single fiber strain stiffening in fibrin networks., Abstract for the 75th Annual Meeting of the Southeastern Section of APS, Raleigh, NC., Oct. 20 - Nov. 1, 2008.