PhIFI Core Facility Site

PhIFI Core Facility
(Phosphor-Imaging/Fluorescence Imaging)
Brody 5S-21

The Typhoon 9410 Imager....	Administrator: Dr. Brett D. Keiper contact: keiperb@ecu.edu
The PhIFI Core Facility at the Brody School of Medicine/ECU houses a fully equipped Amersham/ GE Healthcare Typhoon 9410 Imager for 2D imaging of phosphorescent, chemiliumscent and fluorescent gel and array data. The Phosphor-Imaging/Fluorescence Imaging core facility (PhIFI) is located on the 5th floor in the Brody Building in Room 5S-21. It is available for use by all research laboratories on the East Campus and Brody Medical School of ECU.	Typhoon 9410
	The Typhoon 9410 is capable of detecting, imaging and quantifying five different forms of emission: phosphor autoradiography, chemiluminescence, blue-excited fluorescence, green-excited fluorescence, and red-excited fluorescence. Thus, in addition to radioactivity detection like the old Phosphorimager, it allows direct fluorescence and phosphorescence measurement. All imaging modes have a linear dose response of five orders of magnitude. It can also scan to a resolution of 10 um, making the Typhoon 9410 unique in its ability to image microarrays.

Examples of Routine Typhoon Imager Uses:

•    Radioactivity detection from a blot or dried gel
(³²P,³³P, ³⁵S,¹⁴C, etc.; requires Phosphorimage screen)

•    Chemiluminescent/Fluorescent detection on western blots using ECL+

•    Multiple fluorophore labeled proteins, individual detection (e.g. Cy3 vs Cy5)

•    Fluorescent tag labeled DNA detection (e.g. ROX, TAMRA, etc.)
on wet gels between plates

•    Imaging of slide-mounted DNA or Protein microarrays

•    Protein detection (in gel) by Sypro Ruby stain

	East Carolina University Brett D. Keiper, Ph.D. (Administrator) Dept of Biochemistry & Molecular Biology Brody School of Medicine Greenville, NC 27858 keiperb@ecu.edu	Enhui Hao (Research Asst. III) Dept of Biochemistry & Molecular Biology Brody School of Medicine Greenville, NC 27858 Tel. 744-2693 haoe@ecu.edu	bd keiper 5/06 The above images are from the Amersham/GE Website: (www5.amershambiosciences.com)