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PhIFI Core Facility (Phosphor-Imaging/Fluorescence Imaging) Brody 5S-21 |
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| Workstations in the PhIFI Core Facility... |
Administrator: Dr. Brett D. Keiper contact: keiperb@ecu.edu |
Scanner Station Amersham Typhoon 9410 Scanner • Uses 4 independent lasers • Provides 5 wavelengths for excitation • Can be used with most common fluorophores (e.g. Cy dyes, FITC, GFP, Sybr) Dell GX270 dedicated to scanner workstation • Networked on INTRA for access to lab computers • CDRW/DVDRW drive for burning data to disks • Access to Pirate Drive (for those with accounts) for data backup Data Analysis Station Dell GX110 workstation • Networked on INTRA for file management from scanner • Contains ImageQuant TL and ImageQuant 5.0 software • CDRW drive for burning data to disks New Plastic-backed 8” x 10” Phosphorimage Screens Image Eraser for Phosphorimage Screens Software Download (ImageQuant TL) and License Server • Download ImageQuant TL to your local computer (prior PI registration required) • Visualize, optimize, quantitate and export images • Software runs on your networked Windows PC • Access to INTRA network required for License verificationBrody School of Medicine/ ECU has been awarded grants from the North Carolina Biotechnology Center (NCBC) and the Division of Research and Graduate Studies to set up a Core Facility for 2D imaging of phosphorescent, chemiliumscent and fluorescent gel and array data. Grant Funding was used to purchase a fully equipped Amersham/ GE Healthcare Typhoon 9410 Imager. The Phosphor-Imaging/Fluorescence Imaging core facility (PhIFI) is now functioning on the 5th floor in the Brody Building in the Department of Biochemistry and Molecular Biology in Room 5S-21. It is available for use by all research laboratories on the East Campus and Brody Medical School of ECU. |
Typhoon 9410 |
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East Carolina University
Brett D. Keiper, Ph.D. (Administrator) Dept of Biochemistry & Molecular Biology Brody School of Medicine Greenville, NC 27858 keiperb@ecu.edu |
Enhui Hao (Research Asst. III) Dept of Biochemistry & Molecular Biology Brody School of Medicine Greenville, NC 27858 Tel. 744-2693 haoe@ecu.edu |
bd keiper 5/06 The above images are from the Amersham/GE Website:
(www5.amershambiosciences.com) |