Research

Resources

ECHI 4th floor (Bench)
- Benches (4400-7/8)
- Cell/Virus culture room (4306/8)
- Human cell culture room (4301)
- Radioisotope room (4310)
- Fumehood alcove (4402)
- Animal procedure room (4313F)
- Animal holding room (4313A/B/D/E)
- Imaging rooms (4305/7/8) (2-photon and confocal)
- Bacterial room (4128)
- Core equipment room 1 (4129) (QPCR, Seahorse, LI-COR, cell counter, NanoDrops)
- Core equipment room 2 (4127) (Ultracentrifuge, High-speed centrifuge, HPLC, GC)
- Oroboros O2k/Fluorometer (4400-39/40)
- Dark room (4303)
- Clinical blood analysis room (4301)
- Liquid nitrogen room (4301A)
- Washer room (4312)
- Autoclave room (4221)

ECHI 2nd/3rd floor (Clinic)
- Procedure room (2377)
- Sample preparation room (2379)
- Consenting room (2262)
- Exercise Testing room (3000)

LSB 1st floor (Animal facility)
- Animal Holding room (034)
- Barrier Holding room (022)
- Cold room (028)

Brody basement (Animal facility)
- Mouse metabolic analyses room (GW57) (TSE metabolic cages, Echo-MRI, treadmills)
- In vivo imaging room (IVIS Lumina XR-high resolution digital X-ray, bioluminescence and fluorescence)

Ward sports medicine and FITT buildings (Kinesiology)
- Aerobic exercise equipment
- Resistance exercise equipment
- Dual-enery x-ray absorptiometer room
- Quinton 12-lead EKG exercise testing system
- Parvomedics metabolic measuring systems
- Computer controlled environmental chamber
- Wet lab

Funding Sources

K01 DK095774 (PI: Funai), 8/1/13-5/31/17
R03 DK109888 (PI: Funai), 7/1/16-6/30/18
16POST30980047 (PI: Heden), 7/1/16-6/30/18
ECU Startup Fund (PI: Funai), 7/1/13-6/30/18
CLRP DK RWXE2148 (PI: Heden), 7/1/16-6/30/17


Techniques

Animal Procedures
- Glucose/Insulin/Pyruvate tolerance tests
- VO2, RQ, food intake, activity monitoring
- Body composition measurement
- Exercise treadmill testing (high-intensity or endurance)
- Cold tolerance test/Thermoneutrality
- Parabiosis
- Hyperinsulinemic-euglycemic clamp
- 2DG uptake/3MG transport in isolated skeletal muscle
- In vivo insulin injection
- Blood analysis
- Ex vivo muscle contraction
- Ex vivo O2 consumption
- In vivo muscle electroporation
- Muscle fiber permeabilization
- Oroboros O2k oxygraphs
- Seahorse Bioanalyzers
- Primary muscle cell isolation
- Synthesis of genetically-modified mouse
- Mouse genotyping/breeding

Clinical Procedures
- Blood analysis
- Indirect calorimetry
- VO2 max test
- IVGTT
- Hyperinsulinemic-euglycemic clamp
- Muscle biopsy
- Adipose biopsy
- Primary cell isolation

Cell and Molecular Biology Procedures
- Cell culture
- Bacterial culture
- Lentivirus/adenovirus syntheses
- DNA/RNA isolation
- Quantitative PCR
- RNAseq
- Mass Spectrometry
- Seahorse
- Mitochondrial function assays
- Fatty acid oxidation
- Glucose oxidation
- Calcium uptake assay
- Western blotting
- Immunoprecipitation
- Immunofluorescence
- Immunohistochemistry
- Subcellular fractionation
- Enzymatic activity assays
- Luciferase assay
- HPLC




Brody